- This project is suitable for a PhD student
Project description: Research on human BM has shown that bioactive factors influencing the infant immune system are primarily composed of proteins, essential fatty acids and micronutrients. Furthermore, it was recently reported that 30% of the infant gut microbiome originates from mother’s BM, suggesting that AIP-2 likely influences the composition of BM and transmission of key microbial species to the neonate. Therefore, a comprehensive analysis of AIP-2-shaped BM nutritional and microbiome content is pivotal to understand how AIP-2 induces tolerance in pups.
Using a mouse model of tolerance induction, this project will require the use of bioinformatics to integrate data from microbial 16S rRNA sequencing, mass spectrometry, and nuclear magnetic resonance spectrometry (NMR).
Neonate mice will be treated with AIP-2 supplemented with the most abundant/relevant BM components and their role will be evaluated in a model of allergic asthma.