- Honours or PhD
Aberrant cell growth and division is a hallmark of cancer. A number of checkpoints exist to regulate the passage of cells through cell division cycle to ensure that cells copies their DNA and divide it equally amongst two daughter cells. One of the major interests in our lab is to define these checkpoints at a molecular level in order to understand how cancer cells bypass them. We have identified a novel regulator of mitosis (NRM). Cells depleted of NRM arrest in prometaphase due to an activated spindle checkpoint. These cells are unable to progress through the cell cycle due to a loss of mitotic spindle stability. Recent mass spectrometry results have indicated potential pathways of regulation and possible mechanisms. The project will involve characterisation of the spindle stability defect and the role of NRM in mitosis. The techniques learned will include transfection of mammalian cells, analysis of protein expression, protein localization, protein-protein interaction, knock-down of protein expression using siRNA technology, high-resolution live cell imaging to monitor cell division.
Prof Kum Kum Khanna
Dr Murugan Kalimutho: +61 7 3845 3772, Murugan.Kalimutho@qimrberghofer.edu.au