The QIMR Berghofer Histology Facility is a fully equipped service and research laboratory. The facility caters to the needs of scientists and postgraduate students from the QIMR Berghofer Medical Research Institute as well as external and international institutions.
The unit provides technical services and also trains and consults on matters relating to:
- Routine paraffin and cryo histology
- Special stains
- Immunohistochemistry (Tyramide Signal Amplification) and antibody optimisation
- FISH and CISH labelling
- TMA preparation and high-resolution digitisation of histology slides (e.g. Vectra Imaging System).
The facility stocks a broad selection of commonly used primary antibodies and secondary detection antibodies to label mouse, rat, rabbit, goat, sheep, hamster, guinea pig and chicken primary antibodies with horseradish peroxidase, alkaline phosphatase, Alexa fluorescent or Tyramide (TSA) markers.
CONTACT
Andrew Masel
Manager
T +61 0467 586 939
E Histology@qimrberghofer.edu.au
The facility has a comprehensive range of equipment for paraffin and frozen sample processing and microtomy, automated slide dewaxing and staining.
Equipment at the facility includes:
- Tissue Tek VIP6 AI Tissue Processor
- Excelsior ES tissue processor
- Tissue-Tek TEC paraffin embedding station
- ST5010 Autostainer XL (x2)
- CV5030 Robotic Coverslipper
- RM2235 rotary microtomes
- Slide and cassette writers
- Cryostar NX70 Cryostat
- Ventana Discovery Ultra automated IHC stainer
- Vibratome NVSL
- Beecher Tissue MicroArrayer.
Senior Histology Scientists:
- Sang-Hee Park
- Crystal Chang
- Ashwini Potadar