QIMR Berghofer Analytical Services contain fully equipped service and research laboratories, enabling delivery of high quality and reproducible data. Analytical Services caters to the needs of scientists and postgraduate students from QIMR Berghofer as well as external and international institutions.
The facility provides technical services and also trains and consults on:
- Cell line authentication
- Culture Media Services Unit
- Glassware and waste disposal
CONTACT
Paul Collins
Facility Manager
Human cell line authentication
Human cell line authentication uses Short Tandem Repeats (STR) to validate cell line authenticity. A minimum of 10 markers are used to identify a cell line (conforms to ATCC ASN-0002 standard). A small amount of genomic DNA is needed (~50ng) for authentication. Our experienced staff can also offer advice for problematic allelic spectral interpretations.
Cell line quality control (qc) testing
Research excellence using tissue culture requires that the cultured cells be maintained free of Mycoplasma. We provide a service for the detection of viable Mycoplasma in tissue culture. Our luminescence assay only detects viable/live Mycoplasma. Only small amounts of spent culture media are needed ͠~1mL.
Analytical Services has a comprehensive range of equipment for testing and validating cell lines, the preparation of media and chemical disposal.
Equipment at the facility includes:
- Applied Biosystems 3130XL Genetic Analyser
- Synergy H4
- Qubit 2.0 Fluorometer
- Agilent Bioanalyser 2100
The facility accepts work requests from all sources on a pay-for-service basis. For more information, email the facility manager or staff at analytical.facility@qimrberghofer.edu.au.
Requests are charged according to their type and origin. Charges differ for QIMR Berghofer staff and students, external academic clients and commercial clients.