Dr | Post-Doctoral Researcher+61 7 3362 0387
I am a molecular biologist with >9 years of research experience who is skilled in a variety of in vitro laboratory techniques, MS- based proteomics, bioinformatics analyses and animal procedures. I have completed my PhD working with one of the world leaders in proteomics Prof. Matthias Mann, at the Max Planck Institute in Germany and I am proficient at using a variety of bioinformatics tools on proteomics data to analyze complex datasets. During my postdoctoral tenure, mostly based in Australia, I worked with Prof. David James at the University of Sydney and the Garvan Institute and Dr. Jeffery Gorman at QIMR-B.
I am an integral part of the Sid Faithful laboratory team headed by Prof. Bryan Day at QIMRB, one of the leading brain cancer laboratories within Australia. I have a keen interest in studying the Eph-ephrin signalling system in adult brain cancer.
In 2016, I was awarded a 1-year PdCCRS grant from Cancer Australia to understand the role of the Eph-ephrin system in GBM. The funding enabled me to perform an in-depth proteomic analysis of 13 primary GBM cell lines and highlighted their cell-state heterogeneity (D’Souza et al, Cells, 2020).
I used MS-based proteomics to reveal dynamic signalling events underlying transforming growth factor (TGF)-β signalling (D’Souza et al, Sci. Signal, 2014). It suggested that early TGF-β signalling is a mixture of pro and anti-proliferative signals, which is later tailored to inhibit proliferation. This work was chosen as the cover story and was featured in the journal Science.
I investigated the feasibility of a new method called higher energy collisional dissociation (HCD) for the analysis of phosphopeptides (Nagaraj*, D’Souza* et al, JPR, 2010). The work paved the way for HCD to become the method of choice and has been cited >150 times.