The QIMR Berghofer Proteomics Facility is an expert centre for state-of-the-art proteomics analysis. The experienced team of scientists develop and implement innovative mass spectrometric and bioinformatic methods, for qualitative and quantitative characterisation of proteins, in simple or complex mixtures. The facility team can provide comprehensive standard and specialised proteomic workflows.
- Protein identification
- Intact protein analysis
- Discovery proteomics
- Targeted proteomics
- Data analysis and bioinformatics
Proteomics Services Manager
Electrospray Mass Spectrometers
- LTQ Orbitrap Elite (Thermo Fisher)
- LTQ Orbitrap Velos Pro (Thermo Fisher)
- Orbitrap Fusion Tribrid (Thermo Fisher)
- Triple TOF 5600+ (SCIEX)
MALDI Mass Spectrometer
- MALDI-TOF/TOF Ultraflextreme (Bruker)
- nanoACQUITY UPLC (Waters)
- Prominence HPLC (Shimadzu)
The Proteomics Facility also has a range of gel-based (SDS-PAGE and 2D-IEF-SDS-PAGE) and in-solution (IEF) protein fractionation equipment that can be useful for protein analysis. Staff within the Proteomics Facility have in-house instrumentation and extensive experience in sample preparation methods that may be required for protein analysis. The facility infrastructure includes relevant proteomics servers and software.
The Proteomics Facility accepts work that can include all of the workflows listed above and more on request. Experienced facility scientists can assist with all or part of a research project, from experiment design, sample preparation, on-line or off-line liquid-chromatography and mass spectrometry, through to data processing and bioinformatics.
For enquiries about standard or custom proteomic workflows, please contact Dr Madeleine Headlam at Madeleine.Headlam@qimrberghofer.edu.au
QIMR Berghofer CBCRC Building, Level 4, room CC.39
Proteomics Services Manager: Dr Madeleine Headlam, Madeleine.Headlam@qimrberghofer.edu.au
Senior Project Officers: Dr Marcus Hastie and Dr Keyur Dave
Project Officer: Ashok Rana
Proteomics Bioinformatician: Emma Norris