The QIMR Berghofer DNA Sequencing Facility enables both Next Generation and Sanger sequencing to deliver high quality and reproducible data. This facility caters to the needs of scientists and postgraduate students from QIMR Berghofer as well as external and international institutions.
The facility provides technical services and also trains and consults on matters relating to:
- Sanger (Big Dye) sequencing
- Next Generation Sequencing (NGS)
Contact
Paul Collins
Facility Manager
E analytical.facility@qimrberghofer.edu.au
Big Dye sequencing
Big Dye sequencing is used to:
- Verify PCR amplicons/fragments
- Verify plasmids and cosmids
- Verify genomic DNA
- Provide DNA barcoding for species ID.
Next Generation Sequencing
NGS is the key technology used to catalogue mutations in both DNA and RNA and while it has been a research staple for several years, it is only now starting to make inroads into clinical applications. NGS can be used for resequencing, de novo assembly of genomes or other genetic elements, gene discovery, identification of genome variation (SNP identification, genome rearrangements, insertions and deletions), and more. Qualified staff can produce single or paired ends with read lengths ranging from 100-450bp.
EQUIPMENT
The DNA Sequencing Facility has a comprehensive range of equipment for both standard Sanger sequencing and high throughput sequencing (NGS).
Equipment at the facility includes:
- Applied Biosystems 3130XL Genetic Analyser
- Illumina NextSeq 550 System
- Qubit 2.0 Fluorometer
- Synergy H4
- Agilent Bioanalyser 2100
- Illumina Iscan Array Reader
- Ion Torrent PGM
RESEARCH USAGE
The facility accepts work requests from all sources on a pay-for-service basis. For more information, email the Facility manager or staff at analytical.facility@qimrberghofer.edu.au
Requests are charged according to their type and origin. Charges differ for QIMR Berghofer staff and students, external academic clients and commercial clients.
LOCATION
QIMR Berghofer Central building, Level 8, room 829, and
QIMR Berghofer CBCRC, Level 6
STAFF
Facility Manager: Paul Collins, analytical.facility@qimrberghofer.edu.au
