Every cell within our body is exposed to more than 10 000 events of DNA damage per day. These damaging events can occur from exogenous agents (e.g. UV, ionizing radiation) or endogenously during cellular metabolism or from replication stress during S phase. It is of critical importance that repair pathways are in place to address DNA damage when it occurs, as faulty DNA repair can lead to tumour development. The ATM kinase is one of the key signaling molecules which responds to DNA damage within cells, and it phosphorylates multiple downstream targets to regulate the signalling and co-ordination of repair. We have identified a novel interactor and phosphorylation target of ATM, and this project will characterise the role of this protein in the DNA damage response. This project will involve a number of cell biology and molecular biology techniques, including molecular cloning, mutagenesis, tissue culture, immunofluorescence for detection of DNA damage proteins and western blotting.
Expected outcomes and deliverables
Scholars will gain skills in cell biology, molecular biology and/or animal techniques including tissue culture, immunofluorescence, western blotting, FACS, animal handling and dissections and Xenograft Tumour Models.
- Suitable for students with a background in biology who are looking to undertake Honours or a PhD in the future.