|Grace has a strong instrumentation background. She came to QIMR Berghofer from her position as the Flow Cytometry Facility Manager at Peter MacCallum Cancer Institute in Melbourne. She also worked in clinical neurophysiology at the Alfred Hospital in Melbourne and also in the Neurosurgical Unit at The Maudsley Hospital and Institute of Psychiatry in London. She graduated from RMIT University with a Bachelor of Applied Science in 1990 and completed a Masters in Applied Science in 2011.|
Grace is active with the Australasian Flow Cytometry Group (AFCG) in organising courses and meetings and currently holds position on the AFCG executive committee. Grace is currently an elected Councillor on the Active International Society for Advancement of Cytometry (ISAC). She is currently holds a position on the ISAC education, membership and core managers committees. She is a member and chair of the ISAC associated socities committee.
Senior Flow Cytometry Analyst
|Paula graduated from The University of Queensland with a BSc majoring in Microbiology. After working in diagnostic laboratories in Australia and the UK she joined the Flow Cytometry and Imaging Facility at QIMR Berghofer in 1996.|
In her current role as Senior Flow Cytometry Analyst in the facility, she is focused on supporting scientists that perform world class medical research by providing cell sorting services, giving training on the use of equipment, advising on the design and optimisation of multicolor flow cytometry experiments and assisting with analysis of data.
With her extensive experience Paula is dedicated to providing quality services to the clients of the facility helping them to achieve their goals.
|Nigel completed of his Bachelor of Science (Hons) at University College Dublin. He subsequently moved to Brisbane where he joined the Cancer Unit at QIMR Berghofer under the supervision of Dianne Watters and Martin Lavin. Upon completion of his PhD on cell death he moved to the USA to work with Doug Green at the La Jolla Institute for Allergy and Immunology. At this time he developed an interest in using time lapse microscopy to characterise key biological events in events in cells. He was awarded a Peter Doherty Fellowship in 2001 to move to Peter MacCallum Cancer Centre in Melbourne to work with Joe Trapani. In this position he continued to develop microscopy techniques to characterise cell death by cytotoxic drugs and killer lymphocytes and was awarded an RD Wright Fellowship and a Career Development Fellowship by the NHMRC. He returned to Brisbane in 2009 as an ARC Futures Fellow to set up an independent laboratory at the Mater Medical Research Institute. His laboratory developed new techniques to follow individual cells by time lapse microscopy and harvest these cells for further characterisation. He moved to the Flow Cytometry and Imaging Unit in 2013 to continue to develop the microscopy facility at QIMR Berghofer and assist researchers to find the answers to key issues in Medical Research and help develop better therapies for treatment of major health problems that face Australia and the World.|
Flow Cytometry Analyst
|Michael has been involved with flow cytometry since 1995. After completing of his PhD (trafficking of antigen presenting cells in cutaneous carcinogenesis), Michael completed Postdoc’s at the University of Queensland School of Medicine using flow cytometry to study defective T cell responses in Multiple Sclerosis and at QIMR Berghofer immune responses to human cytomegalovirus. For the past four years he managed the flow cytometry facility for the University of Queensland Diamantina Institute, providing a high speed cell sorting service (MoFlo), maintaining, problem solving the facilities cytometers and providing technical advice to facility users. Michael’s high technical expertise and attention to detail with the ability to repair faults and modify equipment to meet our users requirements is a great asset to QIMR Berghofer.|
|Graeme obtained his PhD in Biochemistry in 1973, from the Medical College of Georgia in Augusta GA, USA. As manager of the flow cytometry laboratory in the Immunology Department at St Vincent’s Hospital in Sydney from 1981 to 1988, he obtained extensive experience in research and clinical flow cytometry. From 1988 to 2006 Graeme worked for Coulter Electronics (10 years) and BD (7 years) as a Flow Cytometry Specialist, involved in training flow cytometry operators, flow cytometry customer applications and technical support. He retired from full time work in December 2006 and now works part time in the flow cytometry laboratory at QIMR Berghofer.|
Flow Cytometry Analyst
|Lucie is experienced in flow cytometry and immunology. She graduated from The University of Nantes in France with a Research Master in Therapeutic Research in 2009. She moved to Brisbane to perform her PhD at the University of Queensland where she studied the immune mediators in allogeneic stem cell transplantation and also in pregnancy-induced microchimerism. She was under the supervision of Associate Professor Kiarash Khosrotehrani at UQCCR, Dr Kelli MacDonald and Professor Geoff Hill in the Bone Marrow Transplantation Laboratory at QIMR Berghofer. Upon completion of her PhD, she joined the Gordon and Jesse Gilmour Leukaemia Research Laboratory (formerly the Translational Leukaemia Research Laboratory) at QIMR Berghofer under the supervision of Dr Steven Lane. She specialised in the autophagy cellular process used by leukaemia stem cells. Flow Cytometry and cell sorting constituted a large component in her research where she acquired expertise in the use of different flow cytometry applications. She moved to the Flow cytometry and Imaging facility in July 2015 where she now utilizes her skills in cell sorting and flow cytometry, training and assay development to QIMR Berghofer researchers as well as external clients using the facility.|
Tam Hong Nguyen
|Tam obtained his PhD (molecular and cell biology) from Griffith University in 2007. He then moved to Fred Meunier’s lab at the Queensland Brain Institute (UQ) to take up a post-doctoral appointment. His research focussed on studying the molecular mechanisms underpinning neuronal synaptic function in both the central and peripheral nervous system using various microscopic techniques. During the course of his post-doctoral research, Tam gained experience and skills in light and fluorescence microscopic techniques including immuno and fluorescence labelling, confocal and time-lapse microscopy. He is also familiar with image analysis techniques and the use of image analysis software such as ImageJ, Imaris and Volocity. Tam joined the Flow Cytometry and Imaging Facility at QIMR Berghofer as a Microscopy Analyst in 2014 and is dedicated to providing efficient and quality assistance to QIMR Berghofer researchers for their microscopy and imaging needs, including instrument training, technical advice and image analysis options.|
Flow Cytometry Analyst
|Amanda has extensive experience in both flow cytometry and imaging. Amanda completed her BSc (Hons) in Biological Sciences (1999) and her PhD (2003) at the University of Edinburgh in conjunction with the Moredun Research institute. She then moved to QIMR Berghofer to take up a post-doctoral appointment with Professor Christian Engwerda, defining immune mechanisms in a murine model of visceral leishmaniasis. During this time she developed extensive skills and interests in flow cytometry and cell sorting.|
Amanda then took up a senior postdoctoral position in the laboratory of Professor Jennifer Stow at the Institute for Molecular Biosciences, University of Queensland, studying fundamental mechanisms of macrophage biology, including intracellular trafficking pathways used for cytokine secretion and phagocytosis. Amanda used this postdoctoral period to develop a strong skill set in imaging including deconvolution microscopy, confocal microscopy, live- and fixed-cell imaging, TIRF and FRET. Following on from this postdoc, Amanda stayed at the IMB to work with Dr Kate Schroder, studying inflammasome biology and IL-1b release from macrophages and neutrophils.
Amanda has 13 years’ postdoctoral experience, during which time she has held her own grant funding and has published 30 papers. Amanda moved back to QIMR Berghofer in 2016 to take up a position in the flow cytometry and imaging facility where she has been further developing her skill set, supporting research at the institute, and performing work for contracted clinical trials within the facility.