Fluorescence-activated cell sorting is a specialised type of flow cytometry which provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. As a scientific instrument, it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.
Equipment and costs
Moflo High Speed Cell Sorter
The sorter was installed in QIMR Berghofer in 2005 and is configured with three excitation lasers and nine fluorescent detectors. It was upgraded to a Moflo XDP in June 2012.
FACS ARIA III Cell SorterI
The is configured with four excitation lasers and 18 fluorescent detectors, it was upgraded to a FACS ARIA III in May 2013.
This sorter is housed in a Class 1 type enclosure any biohazard material including all human samples is sorted on this instrument.
For all cost enquiries, please contact Facility Manager Grace Chojnowski.
Making a Booking
Offline analysis software available
There are five offline computers (Mac and PC) with the following software available:
- Cell Quest Pro.
- Modfit cell cycle software (Mac and PC version).
- Flow JO.
- FCS Express.
- BD FACS Diva.