The Mosquito Control Laboratory (MCL) manages state-of-the art pathogen and insect containment facilities within an integrated building that includes animal houses and laboratories. This gives the MCL the capacity to undertake studies on all aspects of vector biology and disease transmission. Our facilities were equipped using a AU$20 million grant from the Queensland Tropical Health Alliance (funded by Queensland State Government) and it is the largest of its kind in the southern hemisphere. It significantly enhances Australia’s ability to respond to emerging vector-borne disease threats in the region and to test novel ideas for mosquito surveillance, control and for interrupting parasite and pathogen transmission. A major remit of the refurbished MCL is to exploit this unique facility to its full potential through building strong collaborative links with parasitology, virology and vector biology laboratories throughout the world. The MCL has permission to hold a number of exotic mosquito strains (and is currently the only Australian facility licensed to rear Aedes albopictus). The laboratory routinely conducts vector competence experiments on local and exotic mosquito strains, including infections with human malaria and arboviruses. Associate Professor Greg Devine is the current president of the Mosquito and Arbovirus Research Committee (MARC) which supports operational research on the mosquito and disease control issues that are prioritised by government, industry and research organisations in Queensland.
Locally, the MCL has access to real-world mosquito-virus transmission systems through a number of vectors and associated alphaviruses (including Ross River and Barmah Forest). Aedes aegypti and the annual dengue outbreaks that occur in the north of the state present further opportunities for internationally-relevant control and transmission studies. Historically, the MCL has had close ties to field sites in Thailand and Vietnam and is strengthening links to Europe, East Africa and Papua New Guinea.
Current Studentships (2014 – 2018)
- Silvia Ciocchetta – biology of exotic mosquitoes (with Istituto Zooprofilattico Sperimentale delle Venezie).
- Brendan Trewin – invasion risks (with CSIRO and UQ).
- Lisa Rigby – environment, adaptation and disease transmission (AAMI and UQ).
- AID scholarship (TBC) – local Wolbachia (with UQ).
Group Leader: Dr Greg Devine
- Dr Leon Hugo, Perpetual JT Wilson Fellow
- Dr Jonathan Darbro, Research Officer
White MT, Lwetoijera D, Marshall J, Caron-Lormier G, Bohan DA, Denholm I, Devine GJ (2014) Negative cross resistance in co-treated bednets: a practicable means of restoring pyrethroid-susceptibility to vectors of malaria. PLoS One in press.
Hugo LE, Jeffery JA, Trewin BJ, Wockner LF, Thi Yen N, Le NH, Nghia le T, Hine E, Ryan PA, Kay BH (2014) Adult survivorship of the dengue mosquito Aedes aegypti varies seasonally in central Vietnam. PLoS Negl Trop Dis 8:e2669.
Sikulu MT, Majambere S, Khatib BO, Ali AS, Hugo LE, Dowell FE (2014) Using a near-infrared spectrometer to estimate the age of anopheles mosquitoes exposed to pyrethroids. PLoS One 9:e90657.
Ritchie SA, Devine GJ (2013) Confusion, knock-down and kill of Aedes aegypti using metofluthrin in domestic settings: a powerful tool to prevent dengue transmission? Parasites & Vectors 6.
Hugo LE, Monkman J, Dave KA, Wockner LF, Birrell GW, Norris EL, Kienzle VJ, Sikulu MT, Ryan PA, Gorman JJ, Kay BH (2013) Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission. PLoS One 8.
Trewin BJ, Kay BH, Darbro JM, Hurst TP (2013) Increased container-breeding mosquito risk owing to drought-induced changes in water harvesting and storage in Brisbane, Australia. International Health 5:251-258.
Darbro JM, Johnson PH, Thomas MB, Ritchie SA, Kay BH, Ryan PA (2012) Effects of Beauveria bassiana on survival, blood-feeding success, and fecundity of Aedes aegypti in laboratory and semi-field conditions. American Journal Tropical Medicine and Hygiene 86:656-664.
Sikulu M, Killeen GF, Hugo LE, Ryan PA, Dowell KM, Wirtz RA, Moore SJ, Dowell FE (2010) Near-infrared spectroscopy as a complementary age grading and species identification tool for African malaria vectors. Parasites & Vectors 3:49.
Devine GJ, Killeen GF (2010) The potential of a new larviciding method for the control of malaria vectors. Malaria Journal 9.
Van Uitregt, V., Hurst, T.P., Wilson, R.S. 2012. Greater costs of inducible behavioural defences at cooler temperatures in larvae of the mosquito, Aedes notoscriptus. Evol Ecol. In press
Hurst, T.P., Ryan, P.A., Kay, B.H. 2012. Efficacy of residual insecticide, Biflex AquaMax applied as barrier treatments for managing mosquito populations in suburban residential properties in southeast Queensland. J Med Entomol. In press
Jeffery, J.A.L., Clements, A.C.A., Nguyen, Y.T., Nguyen, L.H., Tran, S.H., Le, N.T., Vu, N.S., Ryan, P.A., Kay, B.H. 2012. Water level flux in household containers in Vietnam—a key determinant of Aedes aegypti populations dynamics. PLoS One7: 7.
Hurst, T.P., Pittman, G., O’Neill, S.L., Ryan, P.A., Nguyen, H.L., Kay, B.H. 2012. Impacts of Wolbachia infection on predator prey relationships: evaluating survival and horizontal transfer between wMelPop infected Aedes aegypti and its predators. J Med Entomol 49: 624-630.
Darbro, J.M., Johnson, P.H., Thomas, M.B., Ritchie, S.A., Kay, B.H., Ryan, P.A. 2012. Effects of Beauveria bassiana on survival, blood-feeding success and fecundity of Aedes aegypti in laboratory and semi-field conditions. Am J Trop Med Hyg86: 656-664.
Vu, S.N., Nguyen, T.Y., Hoang, M.D., Tran, C.T., Vu, T.T., Nguyen, H.L., Le, H.S., Luu, L.L., Vu, T.Q.H., Ly, H.K.K., Huynh, T.T.T., Lam, L.Z.L., Kutcher, S.C., Aaskov, J.G., Jeffery, J.A.L., Ryan, P.A., Kay, B.H. 2012. Community-based control ofAedes aegypti by using Mesocyclops in southern Vietnam. Am J Trop Med Hyg 86: 850-859.
van Uitregt, V.O., Hurst, T.P., Wilson, W.S. 2012. Reduced size and starvation resistance in adult mosquitoes, Aedes notoscriptus, exposed to predation cues as larvae. J Animal Ecol 81: 108-115.
Hoffmann, A.A., Montgomery, B.L., Popovici, J., Iturbe-Ormaiexe, I., Johnson, P.H., Muzzi, F., Greenfield, M., Durkan, M., Leong, Y.S., Dong, Y., Cook, H., Axford, J., Callahan, A.G., Kenny, N., Omodei, C., McGraw, E.A., Ryan, P.A., Ritchie, S.A., Turelli, M., O’Neill, S.L. 2011. Successful establishment of Wolbachia in Aedes populations to prevent dengue transmission. Nature 476: 454-457.
Nguyen, L.A.P., Clements, A.C.A., Jeffery, J.A.L., Nguyen, T.Y., Vu, S.N., Vaughan, G., Shinkfield, R., Kutcher, S.C., Gatton, M.L., Kay, B.H., Ryan, P.A. 2011. Abundance and prevalence of Aedes aegypti immatures and relationships with household water storage in rural areas in southern Viet Nam. Internatl Health 3: 115-125.
Darbro, J.M., Graham, R.I., Kay, B.H., Ryan, P.A., Thomas, M.B. 2011. Evaluation of entomopathogenic fungi as potential biological control agents of the dengue mosquito, Aedes aegypti, (Diptera: Culicidae). Biocont Sci Tech 21: 1027-1047.
Sikulu, M., Dowell, K.M., Hugo, L.E., Wirtz, R.A., Michel K, Peiris K.H., Moore S., Killeen G. F., Dowell F. E. 2011. Evaluating RNAlater(R) as a preservative for using near-infrared spectroscopy to predict Anopheles gambiae age and species. Malar J 10: 186.
Pelecanos, A.M., Ryan, P.A., Gatton, M.L. 2010. Outbreak detection algorithms for seasonal disease data: a case study using Ross River virus disease. BMC Med Inform Decis Mak 10: 74.
Hurst, T.P., Kay, B.H., Brown, M.D., Ryan, P.A. 2010. Melanotaenia duboulayi influence oviposition site selection by Culex annulirostris (Diptera: Culicideae) and Aedes notoscriptus (Diptera: Culicidae) but not Culex quinquefasciatus (Diptera: Culicidae). Environ Entomol 39: 545-551.
Hugo, L.E., Kay, B.H., O’Neill, S.L., Ryan, P.A. 2010. Investigation of environmental influences on a transcriptional assay for the prediction of age of Aedes aegypti (Diptera: Culicidae) mosquitoes. J Med Entomol 47: 1044-1052.
Tran, H.P., Adams, J., Jeffery, J.A.L., Nguyen, Y.T., Vu, N.S., Kutcher, S.C., Kay, B.H., Ryan, P.A. 2010. Householder perspectives and preferences on water storage and use, with reference to dengue, in the Mekong delta, southern Vietnam. Internatl Health 2: 136-142.
Kay, B.H., Tran, T.T.H., Nguyen, H.L., Tran, M.Q., Vu, S.N., Phan, V.D.H., Nguyen, T.Y., Hill, P.S., Vos, T., Ryan, P.A. 2010. Sustainability and cost of a community-based strategy against Aedes aegypti in northern and central Vietnam. Am J Trop Med Hyg 82: 822-830.
Hugo, L. E., Cook, P.E., Johnson, P. H., Rapley, L.P., Kay, B.H., Ryan, P.A., Ritchie, S.A., O’Neill, S.L.. 2010. Field validation of a transcriptional assay for the prediction of age of uncaged Aedes aegypti mosquitoes in Northern Australia. PLoS Negl Trop Dis 4: e608
Sikulu, M., G., Killeen, G.F., Hugo, L.E., Ryan, P.A., Dowell, K.M., Wirtz, R.A., Moore, S.J., Dowell, F.E.. 2010. Near-infrared spectroscopy as a complementary age grading and species identification tool for African malaria vectors. Parasit Vectors 3: 49
Jeffery, J.A.L., Nguyen, T.Y., Vu, S.N., Le, T.N., Hoffmann, A.A., Kay, B.H., Ryan, P.A. 2009. Characterising the Aedes aegypti population in a Vietnamese village in preparationg for a Wolbachia-based mosquito control strategy to eliminate dengue. PLoS Negl Trop Dis 3:11.
Tran, T.T.H., Hill, P.S., Kay, B.H., Tran, M.Q. 2009. Development of a framework for evaluating the sustainability of community-based dengue control projects. Am J Trop Med Hyg 80: 312-218.
Russell, T.L., Gatton, M.L., Ryan, P.A., Kay, B.H. 2009. Quality assurance of aerial applications of larvicides for mosquito control: effect of granule and catch tray size on field monitoring programs. J Econ Entomol 102: 507-514.
Russell, T.L., Kay, B.H., Skilleter G.A. 2009. Environmental effects of mosquito insecticides on saltmarsh invertebrate fauna. Aquat Biol 6: 77-90.
Moreira, L.A., Iturbe-Ormaitxe, I., Jeffery, J.A., Lu, G., Pyke, A.T., Hedges, L.M., Rocha, B.C., Hall-Mendelin, S., Day, A., Riegler, M., Hugo, L.E., Johnson, K.N., Kay, B.H., McGraw, E.A., van den Hurk, A.F., Ryan, P.A., O’Neill, S.L. 2009. A Wolbachia symbiont in Aedes aegypti limits infection with dengue, chikungunya and Plasmodium. Cell 139: 1268-1278.
Clements, A.C.A., Barnett A.G., Cheng, Z.W., Snow, R.W., Zhou, H.N. 2009. Space-time variation of malaria incidence in Yunnan province, China. Malaria J 8: 180.
Hu, W., Clements, A., Williams, G., Tong, S. 2009. Dengue fever and El Niño-Southern Oscillation in Queensland, Australia: a time series predictive model. Occup Environ Med 67: 305-311.
Hugo, L. E., Quick Miles, S., Kay, B.H., Ryan, P.A.. 2008. Evaluations of mosquito age grading techniques based on morphological changes. J Med Entomol 45: 353-369.
Russell, T.L., Kay, B.H. 2008. Biologically based insectides for the control of immature Australian mosquitoes: a review. Aus J Entomol 47 232-242.
Kay, B.H., Watson, T.M., Ryan, P.A. 2008. Definition of productive Aedes notoscriptus (Diptera: Culicidae) habitats in western Brisbane, and a strategy for their control. Aus J Entomol 47: 142-148.
Kay, B.H. 2008. Biological control of dengue vectors: promises from the past. In Halstead, S. (Ed.) Dengue. Tropical Medicine: Science and Practice 5. Imperial College Press, London.
Cook, P.E., Hugo, L.E., Iturbe-Ormaetxe, I., Williams, C.R., Chenoweth, S.F., Ritchie, S.A., Ryan, P.A., Kay, B.H., Blows, M.W., O’Neill, S.L. 2007. Predicting the age of mosquitoes using transcriptional profiles. Nature Protocols 2: 2796-2806.
Knox, T.B., Nguyen, T.Y., Vu, S.N., Gatton, M.L. , Kay, B.H., Ryan, P.A. 2007. Critical evaluation of quantitative sampling methods for Aedes aegypti (Diptera: Culicidae) immatures in water storage containers in Vietnam. J Med Entomol 44: 192-204.
Kay, B.H., Boyd, A.M., Ryan, P.A., Hall, R.A. 2007. Mosquito feeding patterns and natural infection of vertebrates with Ross River and Barmah Forest viruses in Brisbane, Australia. Am J Trop Med Hyg 76: 417-423.
Hurst, T.P., B.H. Kay, P.A. Ryan, M.D. Brown. 2007. Sublethal effects of mosquito larvicides on swimming performance of larvivorous fish Melanotaenia duboulayi (Atheriniformes: Melanotaeniidae). J Econ Entomol 100: 61-65.
- Ecology and biological control of mosquitoes
- Water filled barriers as potential transport mechanisms for container breeding mosquito species
- Assessment of the relationships between household water storage and water usage patterns and actual and/or potential mosquito breeding sites in Brisbane, Queensland
- Understanding mosquito ageing and survivorship as important factors in the epidemiology of mosquito-borne disease
- Investigating the prevalence of natural Wolbachia bacterial infections in Australian mosquito species
- Developing age grading assays for Anopheles vectors of malaria
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