Researchers from the Division of Immunology and the Australian Centre for Vaccine Development (ACVD) at Queensland Institute of Medical Research (QIMR) and the Bone Marrow Transplant unit at Royal Brisbane and Women’s Hospital (RBWH) have used an experimental treatment to help a bone marrow transplant patient overcome a life threatening infection.
In a world-first, a research team headed by Associate Professor Rajiv Khanna and Professor Geoff Hill have successfully bolstered a transplant recipient’s immunity in a test tube, providing protection against a virus the patient was unable to fight after their transplant. Their own blood cells were grown in the laboratory and returned to the patient, resulting in a full recovery.
“In the case of leukaemia, patients often require a bone marrow stem cell transplant to eradicate the malignancy. As part of this procedure the recipient’s immune system often fails to function properly, leaving them highly susceptible to infection which can be life threatening,” explained Professor Hill.
In this case, the patient had been previously exposed to the human cytomegalovirus (HCMV) – a virus that often infects the lungs and gastrointestinal tract after transplantation. They developed immunity prior to the transplant and the virus had remained dormant. However, after the transplant their new donor immune system was unable to control the infection, in part because it had not been exposed to the virus previously.
The virus took hold and the patient developed severe and life threatening disease that could not be controlled by anti-viral drugs and the patient’s health dramatically declined.
With no other treatments available, experimental immunotherapy was used. T cells are the body’s natural defence system. Different T cells recognise and fight different viruses and bacteria protecting the body against infection. The patient’s T cells were removed and exposed to man-made HCMV proteins. This generated large numbers of healthy T-cells ready and able to fight the virus.
“We believe removing the T cells from the recipient gave them the chance to recuperate and multiply – it allowed the body’s defence system to regroup and re-arm itself against this deadly virus,” said Associate Professor Khanna.
When the bolstered T cells were injected back into the patient they were accepted by their immune system as if they had been produced within the body itself. Positive results were observed after only four weeks of treatment. Six months after treatment the patient had no evidence of disease caused by the virus – the HCMV had stopped replicating – the virus had been beaten.
“What makes this research so exciting is for the first time we have given a patient long term protection against a virus that already existed in their body but which the donor cells were unable to fight – previously this was not thought possible”, said Professor Hill.
“We believe these findings have important implications for the use of immunotherapy for this group of transplant recipients. This treatment has the potential to save the lives of many patients worldwide.”
“However we have to be cautious as this procedure has only been used with one patient. The results are incredibly encouraging and the research teams at QIMR and RBWH are now embarking on a formal prospective clinical trial to thoroughly test this procedure,” explained Professor Hill.
This research was supported by funding from the Leukaemia Foundation of Queensland.
The results were published in the American Journal of Transplantation.